Yuki und sein bester Freund Tetsu schlagen sich mehr schlecht als recht durchs Leben und vor allem leben sie gefährlich! Denn ihre Stadt wird von gefährlichen Straßengangs regiert. Als die beiden einen Einbruch begehen, um die Krankenhaus-Kosten von Yukis kleinem Bruder zu finanzieren, erwischt sie der Anführer der lokalen Yakuza. Er macht Yuki ein Angebot, das dieser nicht ablehnen kann: für einen Besuch pro Woche in seinem Büro will er die Krankenhaus-Kosten übernehmen. Yuki nimmt die Offerte an, ohne an die Folgen des Deals zu denken...
A master class in cooking distills decades of professional experience into four elements: salt, fat, acid, and heat, which when properly applied in the kitchen will produce delicious meals, and includes recipes to put the lessons into practice.
This unique and practical resource provides the most complete and concise summary of underlying principles and approaches to studying nucleic acid structure, including discussion of x-ray crystallography, NMR, molecular modelling, and databases. Its focus is on a survey of structures especially important for biomedical research and pharmacological applications. To aid novices, the book includes an introduction to technical lingo used to describe nucleic acid structure and conformations (roll, slide, twist, buckle, etc.). This completely updated edition features expanded coverage of the latest advances relevant to recognition of DNA and RNA by small molecules and proteins. In particular, the reader will find extensive new discussions on: RNA folding, ribosome structure and antibiotic interactions, DNA quadruplexes, DNA and RNA protein complexes, and short interfering RNA (siRNA). This handy guide ends with a complete list of resources, including relevant online databases and software. - Completely updated with expanded discussion of topics such as RNA folding, ribosome structure and antibiotic interactions, DNA quadruplexes, DNA and RNA protein complexes, and short interfering RNA (siRNA) - Includes a complete list of resources, including relevant online databases and software - Defines technical lingo for novices Review quote: Review of the previous edition ´´Stephen Neidle´s excellent new introductory textbook will prove to be very timely, and provides a calm, balanced and objective study of the major advances.´´ --Crystallography News Table of contents: Methods for Studying Nucleic Acid Structure. The building-blocks of DNA and RNA. DNA Structure as Observed in Fibres and Crystals. DNA-DNA recognition: non-standard and higher-order DNA structures. Principles of small molecule-DNA Recognition. The RNA structural world. Principles of Protein-DNA Recognition.
While cooking at Chez Panisse at the start of her career, Samin Nosrat noticed that amid the chaos of the kitchen there were four key principles that her fellow chefs would always fall back on to make their food better: Salt, Fat, Acid and Heat. Whether you want to balance your vinaigrette, perfectly caramelise your roasted vegetables or braise meltingly tender stews, Samin´s canon of 100 essential recipes and their dozens of variations will teach you how.
This detailed second edition volume expands upon the prior edition by addressing newly emerged technologies as well as improved existing protocols in nucleic acid delivery via nanotechnology. The book addresses topics ranging from chemical synthesis of macromolecules and bioconjugates, novel and established nanoformulations, characterization of these nanoformulations for biophysical, biological and toxicological aspects, and also protocols dealing with application and imaging of such carrier systems in vivo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, Second Edition serves as an ideal guide to researchers seeking to use this vital area of study in their own lab work or to progress knowledge within the field itself.
Massimo Wolke lässt in seinem neuen Werk die Anfangszeiten der Techno & House Bewegung neu aufleben. Dieses Mandalabuch enthält viele Symbole und Anekdoten in Mandalaform, um diese Zeit zu neuem Leben zu erwecken. Massimo Wolke brings you back to the oldskool! This book contains symbols of the great era of dance music between 1988 and 1992 and brings back the whole feeling of the beginning of techno, house & rave with these Rave, Acid Techno & Oldskool Mandalas.
Concise chapters, written by experts in the field, cover a wide spectrum of topics on lipid and membrane formation in microbes (Archaea, Bacteria, eukaryotic microbes). All cells are delimited by a lipid membrane, which provides a crucial boundary in any known form of life. Readers will discover significant chapters on microbial lipid-carrying biomolecules and lipid/membrane-associated structures and processes.